Ọmụmụ ihe anyị na-egosi na Ganoderma lucidum/reishi/lingzhi na-egosi njirimara antitumor na mkpụrụ ndụ osteosarcoma na vitro.Achọpụtara na Ganoderma lucidum na-egbochi uto mkpụrụ ndụ cancer ara na ịkwaga site na ibelata akara Wnt/β-catenin.Ọ na-egbochi ọrịa cancer akpa ume site na nkwụsị nke nrapagidesi ike na ntinye nke MDM2-mediated Slug deradation.Ganoderma lucidum na-egbochi ọrịa ara ara site na ibelata ụzọ PI3K/AKT/mTOR, Ganoderma lucidum na-arụ ọrụ antitumor n'ime mkpụrụ ndụ leukemia siri ike site na igbochi ụzọ MAPK.
CCK-8 na nyocha nhazi nke colony, maka nyochaa mmetụta nke Ganoderma lucidum na osteosarcoma cell line viability na proliferation, gosiri na Ganoderma lucidum na-egbochi mgbasa nke MG63 na U2-OS mkpụrụ ndụ n'oge na ntinye uche, ma na-ebelata. ikike nke mkpụrụ ndụ ịchịkọta.
Ganoderma lucidum na-akwalite okwu nke mkpụrụ ndụ proapoptotic, na nyocha cytometry na-asọpụta gosiri na apoptosis nke MG63 na U2-OS cell na-abawanye mgbe ọgwụgwọ ya na Ganoderma lucidum gasịrị.Mbugharị cell bụ ihe ndabere nke ụdị omume dịgasị iche iche nke ndu, gụnyere angiogenesis, ọgwụgwọ ọnya, mbufụt, na ọrịa cancer metastasis.Ganoderma lucidum na-ebelata mbugharị na mbuso agha nke ahịrị sel abụọ ahụ ma na-egbochi mmụba, mbugharị, na mbuso agha, na-ebute apoptosis nke mkpụrụ ndụ osteosarcoma.
Aberrant Wnt / β-catenin na-egosi njikọ chiri anya na nhazi, metastasis, na apoptosis nke ọtụtụ ụdị ọrịa cancer, na-emeziwanye akara Wnt / β-catenin na osteosarcoma.
N'ime ọmụmụ ihe a, nyocha nke onye nta akụkọ dual-luciferase gosiri na ọgwụgwọ Ganoderma lucidum na-egbochi CHIR-99021-activated Wnt/β-catenin.Ekwupụtakwara nke a site na ngosipụta anyị na-egbochi ndegharị mkpụrụ ndụ ihe nketa Wnt, dị ka LRP5, β-catenin, cyclin D1, na MMP-9, mgbe ejiri Ganoderma lucidum na-agwọ mkpụrụ ndụ osteosarcoma.
Nnyocha ndị gara aga egosila na nlele ụlọ ọgwụ na LRP5 na-emeziwanye na osteosarcoma n'ihe gbasara anụ ahụ nkịtị, na nkwupụta nke LRP5 na-ejikọta ya na ọrịa metastatic na ndụ na-enweghị ọrịa na-adịghị mma, na-eme ka LRP5 bụrụ ihe mgbaru ọsọ ọgwụgwọ maka osteosarcoma.
β-catenin n'onwe ya bụ isi ebumnuche na ụzọ akara Wnt / β-catenin, na okwu β-catenin na osteosarcoma na-abawanye nke ukwuu.Mgbe β-catenin na-atụgharị n'ime oghere site na cytoplasm, ọ na-eme ka nkwupụta nke mkpụrụ ndụ ihe nketa ya, nke gụnyere cyclin D1, C-Myc, na MMPs.
Myc bụ otu n'ime proto-oncogenes bụ isi ma na-arụ ọrụ dị mkpa n'ịhazi mmegharị, ntụgharị, na mgbochi nke mkpụrụ ndụ ihe nketa. osteosarcoma.
Cyclin D1 bụ ihe dị mkpa na-achịkwa usoro okirikiri cell G1 ma na-eme ka mgbanwe usoro G1/S dịkwuo elu.Mkwupụta nke cyclin D1 gabigara ókè nwere ike ime ka okirikiri cell dị mkpụmkpụ ma kwalite mmụba sel ngwa ngwa n'ụdị etuto dị iche iche.
MMP-2 na MMP-9 bụ stromelysins nke nwere ikike imebi ihe matrix extracellular, akụkụ dị mkpa maka angiogenesis etuto na mbuso agha.
Nke a na-egosi na mkpụrụ ndụ ihe nketa Wnt / β-catenin na-arụ ọrụ dị mkpa na ọganihu osteosarcoma, na igbochi oghere mgbaàmà ndị a nwere ike inwe mmetụta ọgwụgwọ dị egwu.
Mgbe nke ahụ gasịrị, anyị chọpụtara nkwupụta mRNA na protein nke mkpụrụ ndụ ihe nketa metụtara akara Wnt/β-catenin site na PCR na ọdịda ọdịda anyanwụ.Na ahịrị sel abụọ ahụ, Ganoderma lucidum gbochiri okwu nke protein na mkpụrụ ndụ ihe nketa ndị a.Nsonaazụ ndị a na-egosikwa na Ganoderma lucidum na-egbochi akara Wnt/β-catenin site n'ịchụso LRP5, β-catenin, C-Myc, cyclin D1, MMP-2, na MMP-9.
E-cadherin bụ transmembrane glycoprotein egosipụtara n'ọtụtụ ebe na sel epithelial ma na-emezi njikọ n'etiti sel epithelial na sel stromal.Nhichapụ ma ọ bụ mfu nke okwu E-cadherin na-eduga na mfu ma ọ bụ ike ọgwụgwụ nke njikọ dị n'etiti mkpụrụ ndụ tumo, na-eme ka mkpụrụ ndụ tumor nwee ike ịkwaga ngwa ngwa, wee mee ka ụbụrụ ahụ banye, gbasaa, na metastasize.N'ime ọmụmụ ihe a, anyị chọpụtara na Ganoderma lucidum nwere ike imeziwanye E-cadherin, si otú ahụ na-emegide phenotype Wnt / β-catenin-mediated nke mkpụrụ ndụ osteosarcoma.
N'ikpeazụ, nsonaazụ anyị na-egosi na Ganoderma lucidum na-egbochi osteosarcoma Wnt/β-catenin na-eduga na mbelata ọrụ cell osteosarcoma.Nchọpụta ndị a na-egosi na Ganoderma lucidum nwere ike ịbụ onye na-ahụ maka ọgwụgwọ bara uru ma dị irè maka ọgwụgwọ osteosarcoma, ngwaahịa ndị metụtara ya gụnyere.ganoderma lucidum spore mmanụ softgels/reishi spore oil softgels,ganoderma lucidum spore ntụ ntụ/reishi spore ntụ ntụ
Oge nzipu: Eprel-18-2022